PURIFICATION
Development Services
Purification process by FPLC, Global development or step optimization to purify native or recombinant protein
- Extraction and clarification of biomass from bacteria, yeast, eukaryotic cells, animals or plants
- Capture and concentration
- Intermediate steps to separate the protein of interest from its main contaminants
- Final chromatography steps to increase the purity of the target protein
- Purity analysis by reverse phase HPLC
- Structure analysis by comparison reduced / non-reduced electrophoresis and immunoblotting
- Chromatography step optimization
- Development of a complete purification protocol
Production Services
We propose production of protein batches from your biomass (to speed up your research project, to characterize your protein by mass spectrometry, to produce an antigen for generation of antibody tools, to establish ELISA tests, etc…) |