Publications et Références

Notes d’application

N- and O-linked glycoprofiling of ebolavirus GP1.2 glycoproteins

Note 170317

How to release glycans for glycan analysis ?

Note 042616

N-glycan profiling from in gel separated proteins and Maldi Tof MS analysis

Note 081114

Glycomic analysis: Age related differences of N-glycan profiles in Plasma of healthy Human Donors

Note 021914


Plaquette de présentation

New Flyer: Your Service Partner and Expert for Glycan Analysis, glycomics and Characterization of proteins and Peptides



Elizabeth C. Clarke, Amanda L. Collar, Chunyan Ye, Yíngyún Caì, Eduardo Anaya, Derek Rinaldi, Britney Martinez, Sarah Yarborough, Christine Merle, Manfred Theisen, Jiro Wada, Jens H. Kuhn, and Steven B. Bradfute « Production and Purification of Filovirus Glycoproteins in Insect and Mammalian Cell Lines » Sci Rep. 2017; 7: 15091. Published online 2017 Nov 8. doi:  10.1038/s41598-017-15416-3

Collar A.L., Clarke E.C., Anaya E., Merril D., Yarborough S., Anthony S.M., Kuhn J.H., Merle C., Theisen M., Bradfute S.B. Comparison of N- and O-linked glycosylation patterns of ebolavirus glycoproteins (2017) Virology 502 39-47.Publications scientifiques

Boudhraa Z. ,Merle C.,Mazzocut D.,Chezal J.M., Chambon C., Miot Noirault E., Theisen M., Bouchon B.,Degoul F. Characterization of pro-invasive mechanisms and N-terminal cleavage of ANXA1 in melanoma (2014) Arch Dermatol Res DOI 10.1007/s00403-014-1517-z

Fogli, A. , Merle C., Roussel V., Schiffmann R., Ughetto S., Theisen M., Boespflug-Tanguy, O., CSF N-glycan profiles to investigate biomarkers in brain developmental disorders: Application to leukodystrophies related to eIF2B mutation.(2012), PloS ONE Volume 7, Isue 8 e42688.

Theisen, M., ID Parade. (2009), European Biopharmaceutical Review, July 44-49.

Burtin, D., Chabre, H.,Olagnier, B., Didierlaurent, A., Couret, M.N., Comeau, D , Wambre E, Laparra H, Van Overtvelt, L., Montandon, F., Batard, T., Jonval, V., Lorphelin, A., Merle, C., Berrouet ,C., Parry, L., Gomord, V., Van Ree, R, and Moingeon, P.Production of native and modified recombinant Derp1 molecules in tobacco plants. (2009), Clinical and Experimental Allergy, 39, 760-770.

Perret, S., Eble, J.A., Siljander,P., Merle, C., Farndale,R.,W., Theisen, M.& Ruggiero, F. Prolyl-hydroxylation of collagen type I is required for efficient binding to integrin a1b1 and platelet GPVI but not to a2b1. (2003), J. Biol. Chem. 278 (32): 29873-29879.

Mokrzycki-Issartel, N., Bouchon, B., Farrer, S., Berland, P., Laparra, H., Madelmont, J.-C., Theisen, M. A transient tobacco expression system coupled to MALDI-TOF-MS allows validation of the impact of differential targeting on structure and activity of a recombinant therapeutic glycoprotein produced in plants. (2003), FEBS LETTERS, 2003, 552, 170-176.

Samyn-Petit, B., Wajda Dubos, J.P., Chirat, F., Coddeville, B., Demaizieres, G., Farrer, S., Slomianny, M.-C., Theisen, M.,Delannoy, P.Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants. (2003), Eur. J. Biochem., 207, 3235-3242.

Samyn-Petit, B., Gruber, V., Flahaut, C., Wajda-Dubos, JP., Farrer, S., Pons, A., Desmaizieres, G., Slomianny, MC., Theisen, M., Delannoy, P. N-glycosylation potential of maize: The human lactoferrin used as a model. (2002) Glycoconj. J. (7), 519 6 527.

Merle, C., Perret, S., Lacour, T., Jonval, V., Hudaverdian, S., Garrone, R., Ruggiero, F. and Theisen M. Hydroxylated human homotrimeric collagen I in Agrobacterium tumefaciens-mediated transient expression and in transgenic tobacco plant (2002) FEBS Lett. 515, 114-118.

Perret, S., Merle, C., Bernocco, S., Berland, P., Garrone, R., Hulmes, D.J., Theisen, M. and Ruggiero, F. Unhydroxylated triple helical collagen I produced in transgenic plants provides new clues on the role of hydroxyproline in collagen folding and fibril formation (2001) J. Biol. Chem. 276, 43693-43698.

Publications de nos Clients

Ribela M.T.C.P., Damiani R., Silva F.P., Lima E.R., Oliveira J.E., Peroni C.N., Torjesen P.A., Soares C.R., Bartolini P. N-glycoprofiling analysis for carbohydrate composition and site-occupation determination in a poly-glycosylated protein: Human thyroropin of different origins. (2017) Int. J. Mol. Sci. 18 13; doi:10.3390/ijms18020131

Li J., Stoddard T.J., Demorest Z.L., Lovoie P-O., Luo, S. Clasen B.M., Cedrone F., Ray E.E., Coffman A.P., Daulhac A., Yabandith A., Retterath A.J., Mathis L., Voytas D.F., D’Aoust M-A., Zhang F. Multiplex, targeted gene editing in Nicotiana benthamiana for glyco-engeneering and monoclonal antibody production. (2016) Plant Biotechnology Journal 14 533-542

Fould B, Lamamy V, Guenin SP, Ouvry C, Cogé F, Boutin JA, Ferry G. Mutagenic analysis in a pure molecular system shows that thioredoxin-interacting protein residue Cys247 is necessary and sufficient for a mixed disulfide formation with thioredoxin. (2012) Protein Sci.  Jul 3. doi: 10.1002/pro.2119.

Olivier S., Jacoby M., Brillon C., Bouletreau S., Mollet T., Nerriere O., Angel A.,(1), Danet S., Souttou B. , Guehenneux F., Gauthier L., Berthomé M., Vi H. ; Beltraminelli N .(1) and Majid Mehtali M. EB66 cell line, a duck embryonic stem cell-derived substrate for the industrial production of therapeutic monoclonal antibodies with enhanced ADCC activity. mAbs 2:4, 405-415; July/August 2010; (2010) Landes Bioscience.

Damiani R., Oliveira J.E., Vorauer-Uhl K., Nunes Peroni C.,Gimbo Vianna E., Bartolini P., Ribela M.T.C.P., Stable expression of a human-like sialylated recombinant thyrotropin in a Chinese hamster ovary cell line expressing a2,6-sialyltransferase Expression and Purification 67 (2009) 7–14.

Giganti A, Rodriguez M, Fould B, Moulharat N, Cogé F, Chomarat P, Galizzi JP, Valet P, Saulnier-Blache JS, Boutin JA, Ferry G. Murine and human autotaxin alpha, beta, and gamma isoforms: gene organization, tissue distribution, and biochemical characterization. Biol Chem. (2008) Mar 21;283(12):7776-89.

Posters Scientifiques

Characterization of annexin A1 role in melanoma dissemination.

Boudhraa Z., Rondepierre F., Trzeciakiewicz A ., Merle C.,Miot-Noirault E., Chezal J.M, Bouchon B., Degoul F.

UMR990 (INSERM/ Auvergne University)/ Proteodynamics, Clermont-Ferrand France

Abstract poster CLARA 20-21 mars (2012) N°25, session Tumor Escape, cell Plasticity and Targeted Therapies

Using wild type CHO cells, improvement of effector functions through MAb engineering analysed by mass spectrometry & by new HTRF® technology

Vermot-Desroches C. (1), F. Chevallier F.(2), Savino M. (2), Merle C.(3), Theisen M.(3), Subiger O.(1), Vuillermoz B.(1), Bourdin L.(1)

iDD biotech (1), Dardilly, France – Cisbio Bioassays(2), Codolet, France – Proteodynamics, Clermont-Ferrand(3), France.

Abstract poster CLARA 20-21mars (2012) N°34, session Tumor Escape, cell Plasticity and Targeted TherapiesAnnexin A1 N-terminal peptde in melanoma dissemination

Boudhraa Z., Rondepierre F., Trzeciakiewicz A ., Merle C.,Miot-Noirault E., Chezal J.M, Bouchon B., Degoul F.Poster

UMR990 (INSERM/ Auvergne University)/ Proteodynamics, Clermont-Ferrand France

Abstract poster (Septembre 2012)